Objective To analyze the diagnostic value of conventional contrast-enhanced ultrasonography and high frame rate contrast-enhanced ultrasonography in differentiating adenomatous polyps from cholesterol polyps. Methods 30 patients with adenomatous polyps and 30 patients with cholesterol polyps underwent conventional contrast-enhanced ultrasonography and high-frame frequency contrast-enhanced ultrasonography before surgery and the diagnostic value was analyzed. Results There was no significant difference in the diagnosis number of adenomatous polyps and cholesterol polyps (P>0.05) between the two types ofultrasoundbut there was significant difference in image features, such as blood flow signal and echo characteristics (P<0.05). In addition, there was no significant difference in the vascular morphology between adenomatous polyps and cholesterol polyps (P>0.05) while there was significant difference in enhancement intensity and gallbladder wall integrity (P<0.05) when conventional contrast-enhanced ultrasoundwas used. Conclusion Conventional contrast-enhanced ultrasound and high frame rate contrast-enhanced ultrasound have certain diagnostic value in differentiating adenomatous polyps and cholesterol polyps. High frame rate contrast- enhanced ultrasound has higher diagnostic value as it can provide more reliable and accurate imaging.